Linkage analysis and you will QTL mapping inside the a great tetraploid russet mapping populace regarding potato

Linkage analysis and you will QTL mapping inside the a great tetraploid russet mapping populace regarding potato

Genome-greater single nucleotide polymorphism (SNP) markers coupled with allele dose pointers enjoys emerged as the a powerful unit to possess learning cutting-edge traits inside expanded autotetraploid potato (Solanum tuberosum L., 2n = 4? = 48). Yet, this approach has been efficiently applied to this new character away from decimal trait loci (QTLs) hidden extremely heritable characteristics like state resistance, however, mostly unexplored for traits which have state-of-the-art habits out of genetics.

Performance

In this study, an F1 tetraploid russet mapping population (162 individuals) was evaluated for multiple quantitative traits over two years and two locations to identify QTLs associated with tuber sugar concentration, processing quality, vine maturity, and other high-value agronomic traits. We report the linkage maps for the 12 potato chromosomes and the QTL location with corresponding genetic models and candidate SNPs explaining the highest phenotypic variation for tuber quality and maturity related traits. Significant QTLs for tuber glucose concentration and tuber fry color were detected on chromosomes 4, 5, 6, 10, and 11. Collectively, these QTLs explained between 24 and 46% of the total phenotypic variation for tuber glucose and fry color, respectively. The QTL on chromosome 10 was associated with apoplastic invertases, with ‘Premier Russet’ contributing the favorable allele for fry processing quality. On chromosome 5, minor-effect QTLs for tuber glucose concentration and fry color co-localized with various major-effect QTLs, including vine maturity, growth habit, tuber shape, early blight (Altenaria tenuis), and Verticillium wilt (Verticillium spp.).

Results

Linkage studies and QTL mapping from inside the a great russet mapping people (A05141) using SNP amount pointers effortlessly understood advantageous alleles and you may applicant SNPs for effectiveness the brand new accumulation away from tuber reducing glucose. These book indicators provides a leading possibility of the improvement out-of tuber handling top quality. Additionally, the brand new advancement of various hereditary activities having attributes that have overlapping QTLs during the maturity locus clearly means a different genetic control.

Record

Having a complex polyploid genetics the fresh new grown autotetraploid potato (Solanum tuberosum L., 2n = 4? = 48) gift ideas novel pressures for linkage mapping and you proceed this link here now can decimal feature loci (QTL) research. This new recent growth of potato single nucleotide polymorphism (SNP) arrays will bring some genome-greater markers used in mapping and you will decimal attribute locus (QTL) investigation [step 1,2,step three,4]bined to your supply of statistical gadgets that allow the use from allele dosage information (we.age., how many copies each and every allele in the confirmed polymorphic locus), it is now you can easily to somewhat improve the electricity regarding SNP recommendations to help you position recombination incidents and to make use of this article on the QTL mapping [5,six,7]. Thus far, this process has been efficiently used to position book QTLs and you can applicant SNPs with the late blight resistance [4, 8], interior temperature necrosis , Potato malware Y , and you can agronomic qualities , but mostly unexplored getting reading running top quality or any other higher-worth faculties having advanced inheritance.

Discover a need for molecular indicators connected with tuber processing high quality qualities to simply help your choice of germplasm for cultivar creativity. Processing quality is basically influenced by tuber glucose articles. The fresh new buildup out-of fructose and you can glucose (reducing sugar) for the potato tubers during cold storage adversely impacts the finish-have fun with quality features. Reducing sugar stuff is an intricate trait controlled by multiple genetic and you can environment circumstances [a dozen, 13]. The state-of-the-art hereditary tissues regarding the feature is similar to the activity of a lot enzymes active in the metabolic paths connecting starch dysfunction and synthesis to help you glucose creation and utilization [14, 15]. Hereditary mapping studies have shown one to fry handling top quality and you may reducing sugars are of this countless brief impression QTLs [sixteen,17,18,19,20]. Soltis-Kalina ainsi que al. understood a couple major QTLs to have chip colour towards chromosomes 1 and you may six inside a beneficial diploid mapping population. Recently, Braun mais aussi al. advertised several QTLs to have effectiveness cool-induced sweetening to the chromosomes cuatro and you will 6 regarding diploid potato. Many of these research has come did toward diploid potato germplasm. Although not, after that studies are needed seriously to top comprehend the hereditary frameworks regarding these traits on tetraploid height for the communities produced by russet-skinned potato types.